Toxoplasma Membrane Inositol Phospholipid Binding Protein TgREMIND Is Essential for Secretory Organelle Function and Host Infection

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.19.533351v1?rss=1 Authors: Houngue, R., Sangare, L. O., Alayi, T. D., Dieng, A., Bitard-Feildel, T., Boulogne, C., Slomianny, C., Atindehou, C. M., Fanou, L. A., Hathout, Y., Saeij, J. P., Callebaut, I., Tomavo, S. Abstract: Apicomplexan parasites have specialized secretory organelles called rhoptries, micronemes, and dense granules that are essential for host infection. Here, we show that TgREMIND, a Toxoplasma gondii protein containing a membrane phospholipid interacting domain, is required for the biogenesis of rhoptries and dense granules. TgREMIND contains a Fes/CIP4 homology-Bin/Amphiphysin/Rvs (F-BAR) domain at the N-terminus, known to promote cell membrane bending, and a novel uncharacterized domain that we named REMIND for regulator of membrane interacting domain at the C-terminus. TgREMIND binds to PIP2 lipid species and both F-BAR and REMIND domains are necessary to ensure proper biological activities in vitro and in cellulo. Conditional depletion of TgREMIND results in the absence of dense granules and abnormal transparent rhoptries, leading to a severe inhibition of parasite motility, host invasion, and dissemination. Thus, our study demonstrates that TgREMIND is essential for the proper functioning of key secretory organelles required for successful infection by Toxoplasma. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC