Ciliary protein CEP290 regulates focal adhesion via microtubule system in non-ciliated cells

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.02.535304v1?rss=1 Authors: Matsuo, K., Nakajima, Y., Shigeta, M., Kobayashi, D., Sakaki, S., Inoue, S., Takeshita, N., Ueyama, A., Nishikawa, K., Saba, R., Yokoyama, T., Yashiro, K. Abstract: Almost all differentiated mammalian cells have primary cilia on their surface. Ciliary dysfunction causes ciliopathy in humans. Centrosomal protein 290 (CEP290) is a ciliary protein that causes ciliopathies, localizes at the cilial base in ciliated cells, whereas it localizes to the centrosome in non-ciliated proliferating cells. The cilia-dependent function of CEP290 has been extensively studied; however, the cilia-independent function, which is likely responsible for the wider phenotypic spectra of CEP290-related ciliopathies, remains largely unknown. Here, we examined cilia-independent functions of CEP290 in non-ciliated cells. Our study showed that Cep290 function loss suppresses microtubule elongation due to microtubule organizing center malfunction. Surprisingly, CEP290 forms a complex with the adenomatous polyposis coli (APC) protein encoded by the adenomatous polyposis coli gene. The APC-CEP290 complex exists in the centrosome and on microtubule fibers. Notably, the reduced focal adhesion formation is likely responsible for the Cep290 mutant phenotypes, including impaired directed cell migration, shrunken cell shape, and reduced adhesive capacity to the extracellular matrix. The APC-CEP290 complex is consistently important for transporting a focal adhesion molecule, paxillin, to focal adhesions in non-ciliated cells. Thus, our findings provide a novel platform to better understand the ciliopathies. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC