Mapping rhodopsin trafficking in rod photoreceptors with quantitative super-resolution microscopy

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.20.537413v1?rss=1 Authors: Haggerty, K. N., Eshelman, S. C., Sexton, L. A., Frimpong, E., Rogers, L. M., Agosto, M. A., Robichaux, M. A. Abstract: Photoreceptor cells in the vertebrate retina have a highly compartmentalized morphology for efficient long-term phototransduction. Rhodopsin, the visual pigment in rod photoreceptors, is densely packaged into the rod outer segment sensory cilium and continuously renewed through essential synthesis and trafficking pathways housed in the rod inner segment. Despite the importance of this region for rod health and maintenance, the subcellular organization of rhodopsin and its trafficking regulators in the mammalian rod inner segment remain undefined. We used super-resolution fluorescence microscopy with optimized retinal immunolabeling techniques to perform a single molecule localization analysis of rhodopsin in the inner segments of mouse rods. We found that a significant fraction of rhodopsin molecules was localized at the plasma membrane in an even distribution along the entire length of the inner segment, where markers of transport vesicles also colocalized. Thus, our results collectively establish a model of rhodopsin trafficking through the inner segment plasma membrane as an essential subcellular pathway in mouse rod photoreceptors. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC