Polarized localization of kinesin-1 and RIC-7 drives axonal mitochondria anterograde transport

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.12.548706v1?rss=1 Authors: Wu, Y., Ding, C., Weinreb, A., Manning, L., Swaim, G., Yogev, S., Colon-Ramos, D., Hammarlund, M. Abstract: Mitochondria transport is crucial for mitochondria distribution in axons and is mediated by kinesin-1-based anterograde and dynein-based retrograde motor complexes. While Miro and Milton/TRAK were identified as key adaptors between mitochondria and kinesin-1, recent studies suggest the presence of additional mechanisms. In C. elegans, ric-7 is the only single gene described so far, other than kinesin-1, that is absolutely required for axonal mitochondria localization. Using CRISPR engineering in C. elegans, we find that Miro is important but is not essential for anterograde traffic, whereas it is required for retrograde traffic. Both the endogenous RIC-7 and kinesin-1 act at the leading end to transport mitochondria anterogradely. RIC-7 recruitment to mitochondria requires its N-terminal domain and partially relies on MIRO-1, whereas RIC-7 accumulation at the leading end depends on its disordered region, kinesin-1 and metaxin2. We conclude that polarized transport complexes containing kinesin-1 and RIC-7 form at the leading edge of mitochondria, and that these complexes are required for anterograde axonal transport. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC