An mTOR/RNA pol I axis shapes chromatin architecture in response to fasting

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.22.550032v1?rss=1 Authors: Al-Refaie, N., Padovani, F., Binando, F., Hornung, J., Zhao, Q., Towbin, B. D., Sarinay Cenik, E., Stroustrup, N., Schmoller, K. M., Cabianca, D. S. Abstract: Chromatin architecture is a fundamental mediator of genome function. Fasting is a major environmental cue across the animal kingdom. Yet, how it impacts on 3D genome organization is unknown. Here, we show that fasting induces a tissue-specific, reversible and large-scale spatial reorganization of chromatin in C. elegans. This fasting-induced 3D genome reorganization requires inhibition of the nutrient-sensing mTOR pathway, a major regulator of ribosome biogenesis. Remarkably, loss of transcription by RNA Pol I, but not RNA Pol II nor Pol III, induces a similar 3D genome reorganization in fed animals, and prevents the restoration of the fed-state architecture upon restoring nutrients to fasted animals. Our work documents the first large-scale chromatin reorganization triggered by fasting and reveals that mTOR and RNA Pol I shape genome architecture in response to nutrients. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC